Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), APC-eFluor 780, eBioscience

Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), APC-eFluor 780

Applications and Tested Dilutions

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• Biotin
• Brilliant UV 496 / 563 / 615 / 661 / 737
• Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue, Red, Yellow Series
• PE, PE-Cyanine5 / 5.5 / 7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 780
• Request custom conjugation available

Product Specific Information

The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain, along with gamma and delta, is essential for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells. Binding of 145-2C11 to TCR initiates intracellular signaling leading to activation, proliferation, or apoptosis depending on conditions.

Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse splenocytes at ≤1 µg/test (100 µL volume, 10⁵–10⁸ cells/test). Antibody should be titrated for optimal performance.

Fluorophore Information:

• Excitation: 633–647 nm
• Emission: 780 nm
• Laser: Red Laser
• Light sensitivity: Protect from light (photo-induced oxidation)
• Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD3 complex transduces antigen-recognition signals into the cytoplasm of T cells and regulates TCR surface expression. CD3 subunits (gamma, delta, epsilon, zeta) are members of the immunoglobulin superfamily encoded on chromosome 11. They associate with cytoplasmic signal transduction molecules via tyrosine-based motifs. CD3 is expressed on most T lymphocytes and thymocytes but not on B or NK cells. Defects in CD3 genes are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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