Thermo Fisher Scientific Perforin Monoclonal Antibody (dG9 (delta G9)), PerCP-eFluor 710, eBioscience
인간 및 돼지 세포에서 perforin 단백질을 검출하는 마우스 단클론 항체. PerCP-eFluor 710 형광체 결합으로 유세포분석(Flow Cytometry)에 최적화. 세포 내 염색용으로 사전 적정 및 검증 완료. 4°C 암소 보관, 동결 금지.
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Applications
- Flow Cytometry (Flow): 5 µL (0.03 µg)/test
- Tested and validated for intracellular staining and flow cytometric analysis.
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Pig |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), PerCP-eFluor™ 710, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | dG9 (delta G9) |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation/Emission Max | 482/708 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1944475 |
Available Formats
Product Specific Information
The dG9 (delta G9) antibody reacts with human perforin (pore-forming protein, pfp), a cytolytic mediator found in cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin plays a critical role in immune defense against tumors and viral infections.
- Applications Reported: Intracellular staining followed by flow cytometric analysis.
- Applications Tested: Pre-titrated and validated for intracellular staining of normal human peripheral blood cells.
- Use 5 µL (0.03 µg) per test (100 µL final volume).
- Recommended cell range: 10⁵–10⁸ cells/test.
Fluorochrome Information:
- PerCP-eFluor® 710 can substitute for PE-Cy5, PE-Cy5.5, or PerCP-Cy5.5.
- Excitation: 488 nm (Blue laser)
- Emission: 710 nm
- Recommended filters: 685 LP dichroic mirror and 710/40 band pass filter (695/40 acceptable alternative).
Fixation Stability:
PerCP-eFluor® 710 conjugated antibodies remain stable when stained samples are fixed with 2% formaldehyde overnight at 4°C. Evaluate alternative fixation protocols as needed.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Perforin is a major cytolytic protein stored in cytoplasmic granules of cytotoxic lymphocytes. It mediates pore formation in target cell membranes, facilitating immune defense against tumors and viral infections.
- Protein size: 555 amino acids (70–75 kDa)
- Structure: Contains a 21 amino acid signal peptide; homologous to complement component C9.
- Expression: Restricted to killer cell lines, not found in helper T cells or other tumor cells.
- Clinical relevance: Mutations in the perforin gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2).
- Alternative splicing: Multiple transcript variants reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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