Thermo Fisher Scientific Caspase 9 (Cleaved Asp330) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Caspase 9 (cleaved Asp330). The exact sequence is proprietary. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Caspase 9 (cleaved Asp330),uniProtId:P55211-1,ncbiNodeId:9606,antigenRange:416,antigenLength:416,antigenImageFileName:PA5-77889_Caspase_9_cleaved_Asp330_P55211-1_Rabbit.svg,antigenImageFileNamePDP:PA5-77889_Caspase_9_cleaved_Asp330_P55211-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.73 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2735576

Product Specific Information

Positive Control: Jurkat (10 µM Etoposide treatment for 24 hr)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Caspase 9 (Cleaved Asp330) encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.