Thermo Fisher Scientific Phosphotyrosine Monoclonal Antibody (pY20)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

View 48 publications 48 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

-

View 4 publications 4 publications

ELISA (ELISA)

1 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

2-5 µg

View 8 publications 8 publications

Miscellaneous PubMed (Misc)

-

View 19 publications 19 publications

Product Specifications

Species Reactivity

Chemical

Published species

Chemical

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

pY20

Immunogen

Keyhole limpet hemocyanin coupled with equal amounts of phosphotyrosine, glycine, and alanine

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• AP
• HRP
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2532959

Product Specific Information

This antibody detects proteins which contain phosphorylated tyrosine residues. This antibody is known to show cross-reactivity towards phosphothreonine.

Target Information

The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.