Thermo Fisher Scientific Phosphotyrosine Monoclonal Antibody (pY20), Sepharose 4B

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

25-50 µL / 1 mL of cell lysate

View 5 publications 5 publications

Miscellaneous PubMed (Misc)

-

View 19 publications 19 publications

Product Specifications

Published species

Chemical

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

pY20

Immunogen

Keyhole limpet hemocyanin coupled with equal amounts of phosphotyrosine, glycine, and alanine

Conjugate

Sepharose® 4B Sepharose® 4B Sepharose® 4B

View additional formats

• Unconjugated
• AP
• HRP
• Request custom conjugation

Form

Slurry

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_2532962

Product Specific Information

This monoclonal antibody is highly purified and then conjugated to agarose (4% cross-linked). The conjugate is supplied as 0.5 mL of settled gel in 1 mL of 50% suspension containing 10mM PBS, pH 7.0, 100mM NaCI, and 0.1% proclin.

This antibody is known to show reactivity towards phosphothreonine.

Target Information

The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.