Thermo Fisher Scientific Rat anti-Mouse IgG (H+L) Secondary Antibody, HRP

Applications

Tested Dilution

Western Blot (WB)

1:1,000-1:5,000

Immunocytochemistry (ICC/IF)

1:500-1:1,000

ELISA (ELISA)

1:500-1:1,000

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG1, kappa

Class

Monoclonal

Type

Secondary Antibody

Clone

LO-MG-7, LO-MG-1-2, and LO-MG3-7

Immunogen

Gamma Immunoglobin

Conjugate

HRP HRP HRP

View additional formats

• [Biotin ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Biotin/host/Rat/)
• [FITC](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/FITC/host/Rat/)
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4, with 4mg/mL BSA, 40% glycerol

Contains

0.19% Kathon™ CG

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2532933

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

This monoclonal Rat anti-Mouse IgG cocktail reacts with all subclasses of mouse IgG and exhibits minimal reactivity to mouse IgA, IgM, and light chains. Cross-reactivity has been observed with swine and human IgG.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.