Thermo Fisher Scientific CD54 (ICAM-1) Monoclonal Antibody (eBioKAT-1 (KAT-1, KAT1)), Functional Grade, eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Formats

• Unconjugated
• APC
• PE
• Super Bright 436
• Request custom conjugation

Product Specific Information

Description:
The eBioKAT-1 monoclonal antibody reacts with mouse CD54 (ICAM-1), a 95 kDa member of the immunoglobulin superfamily. CD54 is expressed at low levels on leukocytes and high endothelial venules, with expression increasing in response to inflammatory cytokines. ICAM-1 binds to LFA-1, facilitating T cell transendothelial migration. ICAM-1-deficient mice show reduced leukocyte migration and impaired inflammatory responses. The eBioKAT-1 antibody recognizes a different epitope than YN1/1.7.4.

Applications Reported:

• Flow cytometric analysis
• Immunoprecipitation
• Immunohistology of frozen tissue sections
• Inhibition of in vivo ICAM-1-mediated leukocyte migration

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.5 µg per test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best performance.

Storage and Handling:
Use in a sterile environment.

Filtration:
0.2 µm post-manufacturing filtered.

Purity:

90% (SDS-PAGE)

Endotoxin Level:
<0.001 ng/µg antibody (LAL assay)

Aggregation:
<10% (HPLC)

Target Information

ICAM-1 (CD54) is an 85–110 kDa single-chain type I integral membrane glycoprotein with five extracellular immunoglobulin-like domains, a transmembrane region, and a cytoplasmic domain. It binds to integrins CD11a/CD18 (LFA-1) and CD11b/CD18 (Mac-1), and serves as a receptor for Rhinovirus. ICAM-1 is expressed on activated endothelial cells, epithelial cells, fibroblasts, and various immune cells, with expression increasing during inflammation. Soluble ICAM-1 is detectable in plasma and elevated in inflammatory syndromes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.