Thermo Fisher Scientific CD54 (ICAM-1) Monoclonal Antibody (eBioKAT-1 (KAT-1, KAT1)), Functional Grade, eBioscience
마우스 CD54(ICAM-1)을 인식하는 eBioKAT-1 단클론 항체로, 염증 반응 연구에 적합합니다. Flow cytometry, immunoprecipitation, IHC(F) 등 다양한 응용에 사용 가능하며, 0.5 µg/test로 권장됩니다. Functional grade로 제조되어 세포 기능 분석에 적합합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | – | 1 publication |
| Immunohistochemistry (IHC) | – | 4 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | – |
| Immunocytochemistry (ICC/IF) | – | 2 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | 1 publication |
| Immunoprecipitation (IP) | Assay-dependent | 1 publication |
| Neutralization (Neu) | Assay-dependent | – |
| Functional Assay | Assay-dependent | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioKAT-1 (KAT-1, KAT1) |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
| RRID | AB_2865759 |
Additional Formats
Product Specific Information
Description:
The eBioKAT-1 monoclonal antibody reacts with mouse CD54 (ICAM-1), a 95 kDa member of the immunoglobulin superfamily. CD54 is expressed at low levels on leukocytes and high endothelial venules, with expression increasing in response to inflammatory cytokines. ICAM-1 binds to LFA-1, facilitating T cell transendothelial migration. ICAM-1-deficient mice show reduced leukocyte migration and impaired inflammatory responses. The eBioKAT-1 antibody recognizes a different epitope than YN1/1.7.4.
Applications Reported:
- Flow cytometric analysis
- Immunoprecipitation
- Immunohistology of frozen tissue sections
- Inhibition of in vivo ICAM-1-mediated leukocyte migration
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.5 µg per test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best performance.
Storage and Handling:
Use in a sterile environment.
Filtration:
0.2 µm post-manufacturing filtered.
Purity:
90% (SDS-PAGE)
Endotoxin Level:
<0.001 ng/µg antibody (LAL assay)
Aggregation:
<10% (HPLC)
Target Information
ICAM-1 (CD54) is an 85–110 kDa single-chain type I integral membrane glycoprotein with five extracellular immunoglobulin-like domains, a transmembrane region, and a cytoplasmic domain. It binds to integrins CD11a/CD18 (LFA-1) and CD11b/CD18 (Mac-1), and serves as a receptor for Rhinovirus. ICAM-1 is expressed on activated endothelial cells, epithelial cells, fibroblasts, and various immune cells, with expression increasing during inflammation. Soluble ICAM-1 is detectable in plasma and elevated in inflammatory syndromes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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