Thermo Fisher Scientific CD54 (ICAM-1) Monoclonal Antibody (eBioKAT-1 (KAT-1, KAT1)), Functional Grade, eBioscience
마우스 CD54(ICAM-1)을 인식하는 eBioKAT-1 단클론 항체로, 염증 반응 연구에 적합합니다. Flow cytometry, immunoprecipitation, IHC(F) 등 다양한 응용에 사용 가능하며, 0.5 µg/test로 권장됩니다. Functional grade로 제조되어 세포 기능 분석에 적합합니다.
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD54 (ICAM-1) Monoclonal Antibody (eBioKAT-1 (KAT-1, KAT1)), Functional Grade, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | – | 1 publication |
| Immunohistochemistry (IHC) | – | 4 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | – |
| Immunocytochemistry (ICC/IF) | – | 2 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | 1 publication |
| Immunoprecipitation (IP) | Assay-dependent | 1 publication |
| Neutralization (Neu) | Assay-dependent | – |
| Functional Assay | Assay-dependent | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioKAT-1 (KAT-1, KAT1) |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
| RRID | AB_2865759 |
Additional Formats
Product Specific Information
Description:
The eBioKAT-1 monoclonal antibody reacts with mouse CD54 (ICAM-1), a 95 kDa member of the immunoglobulin superfamily. CD54 is expressed at low levels on leukocytes and high endothelial venules, with expression increasing in response to inflammatory cytokines. ICAM-1 binds to LFA-1, facilitating T cell transendothelial migration. ICAM-1-deficient mice show reduced leukocyte migration and impaired inflammatory responses. The eBioKAT-1 antibody recognizes a different epitope than YN1/1.7.4.
Applications Reported:
- Flow cytometric analysis
- Immunoprecipitation
- Immunohistology of frozen tissue sections
- Inhibition of in vivo ICAM-1-mediated leukocyte migration
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.5 µg per test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best performance.
Storage and Handling:
Use in a sterile environment.
Filtration:
0.2 µm post-manufacturing filtered.
Purity:
90% (SDS-PAGE)
Endotoxin Level:
<0.001 ng/µg antibody (LAL assay)
Aggregation:
<10% (HPLC)
Target Information
ICAM-1 (CD54) is an 85–110 kDa single-chain type I integral membrane glycoprotein with five extracellular immunoglobulin-like domains, a transmembrane region, and a cytoplasmic domain. It binds to integrins CD11a/CD18 (LFA-1) and CD11b/CD18 (Mac-1), and serves as a receptor for Rhinovirus. ICAM-1 is expressed on activated endothelial cells, epithelial cells, fibroblasts, and various immune cells, with expression increasing during inflammation. Soluble ICAM-1 is detectable in plasma and elevated in inflammatory syndromes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.