Thermo Fisher Scientific CD54 (ICAM-1) Monoclonal Antibody (YN1/1.7.4), Functional Grade, eBioscience

Thermo Fisher Scientific CD54 (ICAM-1) Monoclonal Antibody (YN1/1.7.4), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Biotin
• FITC
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 700
• PE
• Request custom conjugation

Product Specific Information

The YN1/1.7.4 monoclonal antibody reacts with mouse CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90–110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes, dendritic cells, and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligands CD11a and CD11b plays an important role in the inflammatory response. The YN1/1.7.4 antibody recognizes a different epitope than the eBioKAT-1 monoclonal antibody.

Applications Reported:

• Flow cytometric analysis
• Blocking of CD54 in functional studies

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes.
Recommended usage ≤ 0.5 µg per test (100 µL final volume).
Cell number: 10⁵–10⁸ cells/test (empirical determination required).
Careful titration recommended for optimal performance.

Storage and Handling:
Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered
Purity: >90% (SDS-PAGE)
Endotoxin Level: <0.001 ng/µg antibody (LAL assay)
Aggregation: <10% (HPLC)

Target Information

ICAM-1 (CD54) is an 85–110 kDa single-chain type I integral membrane glycoprotein with five immunoglobulin superfamily repeats, a transmembrane region, and a cytoplasmic domain. It has seven potential N-linked glycosylation sites and shares sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins CD11a/CD18 (LFA-1) and CD11b/CD18 (Mac-1), and serves as a receptor for Rhinovirus.

ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B and T lymphocytes, monocytes, macrophages, dendritic cells, and neutrophils. Expression increases upon inflammation and is also found in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in plasma and elevated in various inflammatory syndromes.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.