Thermo Fisher Scientific CLEC14A Polyclonal Antibody
Human CLEC14A 단백질을 인식하는 Sheep Polyclonal Antibody로, WB, IHC, ICC, Flow Cytometry에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS 및 trehalose buffer에 보존. 교차 반응성이 낮고 연구용으로 적합.
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Applications 및 Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–15 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 5–15 µg/mL | View 1 publication |
| Flow Cytometry (Flow) | 2.5 µg per million cells | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human CLEC14A (Glu22–Ala397) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2607014 |
Product Specific Information
- In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human CLEC1, CLEC2, CLEC3B, and CLEC10A is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
CLEC-14A (C-type lectin domain family 14 member A), also known as EGFR-5 (epidermal growth factor receptor 5) or CEG1, is a 490 amino acid single-pass type I membrane protein that contains one C-type lectin domain and an EGF-like domain.
The gene encoding CLEC-14A maps to human chromosome 14, which houses over 700 genes and comprises nearly 3.5% of the human genome.
Chromosome 14 encodes the presinilin 1 (PSEN1) gene, associated with Alzheimer’s disease, and the SERPINA1 gene, which when defective leads to alpha1-antitrypsin deficiency causing lung and liver dysfunction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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