Thermo Fisher Scientific ARC Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Raji
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

ARC (also known as NOL3 - Nucleolar protein 3) has two isoforms:

• Isoform 1: Involved in RNA splicing
• Isoform 2: Functions as an apoptosis repressor that blocks multiple modes of cell death

ARC inhibits extrinsic apoptotic pathways by:

1. Interacting with FAS and FADD upon FAS activation, blocking death-inducing signaling complex (DISC) assembly
2. Interacting with CASP8 in a mitochondria localization- and phosphorylation-dependent manner, limiting soluble CASP8 available for DISC-mediated activation

ARC also inhibits intrinsic apoptotic pathways through interactions with BAX, preventing mitochondrial dysfunction and release of pro-apoptotic factors.
Mutations affecting this gene can result in myoclonus, familial cortical disorders.

Safety Information

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.