Thermo Fisher Scientific CD127 Monoclonal Antibody (A7R34), Brilliant Violet 650, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

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Product Specifications

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Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 561
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661 / 737 / 805
• Brilliant Violet 421 / 711
• eFluor 450 / 660
• FITC
• Functional Grade
• NovaFluor Red 700 / 710
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5 / PerCP-eFluor 710
• Super Bright 436 / 645
• Request custom conjugation

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Product Specific Information

The A7R34 monoclonal antibody reacts with mouse CD127, the high-affinity alpha subunit of the mouse IL-7 receptor.
IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+CD8+) thymocytes.
In the periphery, mature T cells express CD127 at low levels.

A7R34 inhibits IL-7 binding to its receptor and is used in in vivo and in vitro studies to elucidate IL-7 roles in T and B cell development and activation.
Binding of A7R34 blocks SB/199 antibody binding, which also recognizes mouse CD127.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometry of mouse splenocytes (≤1.0 µg/test, 100 µL final volume, 10⁵–10⁸ cells/test)

Brilliant Violet™ 650 (BV650) emits at 649 nm and is intended for use on cytometers equipped with a violet (405 nm) laser.
Ensure instrument compatibility before use.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use

• Super Bright Complete Staining Buffer (SB-4401-42)
• or Brilliant Stain Buffer™ (00-4409-75)
  to minimize non-specific interactions.

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples may be stored up to 3 days at 4°C in the dark using

• IC Fixation Buffer (00-8222-49)
• or 1-step Fix/Lyse Solution (00-5333-54)
  Brilliant Violet™ 650 (BV650) is not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 649 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

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Target Information

CD127 (Interleukin-7 receptor, IL-7R) is a glycoprotein involved in lymphopoiesis regulation.
The active receptor is an alpha/gamma chain heterodimer.
The gamma(c) chain also associates with the IL-2 receptor and activates signal transduction, while the alpha chain determines specific signaling events.
CD127 promotes proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells.
Diseases associated with CD127 dysfunction include severe combined immunodeficiency (SCID) and T cell negative/B cell negative/NK positive SCID.

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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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