Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), Brilliant Violet 650, eBioscience

Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), Brilliant Violet 650, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 615, 661, 737
• Brilliant Violet 421, 480, 605, 786
• eFluor 450
• FITC
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

The 2B8 monoclonal antibody reacts with mouse CD117 (c-Kit receptor), also known as Steel factor receptor or stem cell factor receptor.
This 145-kDa tyrosine kinase receptor is expressed by hematopoietic progenitor cells in mouse bone marrow, mast cells, and plays a role in proliferation and differentiation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse bone marrow cells at ≤0.5 µg/test (100 µL final volume). Optimal titration recommended.

Fluorochrome Information:
Brilliant Violet™ 650 (BV650) emits at 649 nm and is intended for use with a violet (405 nm) laser.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Handling Notes:

• Light sensitive; protect from light.
• Fixation compatible with IC Fixation Buffer or 1-step Fix/Lyse Solution (store up to 3 days at 4°C in dark).
• Not compatible with methanol-based fixation.
• Excitation: 407 nm; Emission: 649 nm; Laser: Violet.

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

KIT (c-KIT) is a proto-oncogene encoding a type 3 transmembrane receptor for mast cell growth factor (MGF or SCF).
KIT regulates growth and activation of hematopoietic and non-hematopoietic cells.
Mutations in KIT are linked to gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism.
Upon SCF binding, c-Kit dimerizes and autophosphorylates tyrosine residues, activating Ras/ERK, PI3K, PLC-γ, and JAK/STAT pathways.
Essential for hematopoiesis, stem cell maintenance, and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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