Thermo Fisher Scientific CD4 Monoclonal Antibody (RPA-T4), Alexa Fluor 660, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (RPA-T4), Alexa Fluor 660, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution

• 5 µL (0.125 µg)/test

Species Reactivity

• Human

Host / Isotype

• Mouse / IgG1, kappa

Recommended Isotype Control

• Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 660, eBioscience™

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 561
• Alexa Fluor 647
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 711
• eFluor 450
• eFluor 506
• FITC
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 690
• Pacific Orange
• PE
• PE-Cyanine5
• PE-Cyanine5.5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by thymocytes, a subpopulation of mature T cells (T-helper cells), and at low levels on monocytes. CD4 acts as a receptor for HIV, and RPA-T4 blocks HIV binding and mixed lymphocyte reaction.
RPA-T4 recognizes a different epitope than OKT4 and does not cross-block binding.
Applications Reported: For flow cytometric analysis.
Applications Tested: Pre-diluted and validated for flow cytometric analysis of normal human peripheral blood cells. Recommended use: 5 µL (0.125 µg) per test in 100 µL final volume, with 10^5–10^8 cells/test.
Alexa Fluor 660 emits at 690 nm, suitable for spectral cytometers multiplexed with APC, Alexa Fluor 647, and Alexa Fluor 700.
Excitation: 662 nm; Emission: 690 nm; Laser: Red Laser

Target Information

CD4 antigen is involved in MHC class II recognition and acts as a co-receptor for HIV. It is primarily expressed on T-helper lymphocytes and also found on monocytes, macrophages, and dendritic cells.
Tissue-level expression includes thymus, lymph nodes, tonsils, spleen, and specific regions of brain and gut.
CD4 functions in early T-cell activation via association with T-cell receptor complex and Lck kinase, and may mediate neuronal damage in immune-related CNS diseases.
Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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