Thermo Fisher Scientific CD4 Monoclonal Antibody (RPA-T4), Alexa Fluor 660, eBioscience
인간 CD4 항원에 특이적인 RPA-T4 단클론 항체로, Alexa Fluor 660 형광체가 결합된 제품입니다. Flow Cytometry 분석에 최적화되어 있으며 HIV 수용체 연구에 활용 가능합니다. 고순도 정제 및 안정적인 형광 발현을 제공합니다.
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Thermo Fisher Scientific CD4 Monoclonal Antibody (RPA-T4), Alexa Fluor 660, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.125 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RPA-T4 |
| Conjugate | Alexa Fluor™ 660 |
| Excitation / Emission Max | 663 / 691 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2896238 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 561
- Alexa Fluor 647
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 496 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 711
- eFluor 450 / 506
- FITC
- Functional Grade
- NovaFluor Blue 510 / 585 / 690
- Pacific Orange
- PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5 / PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Custom Conjugation available
Product Specific Information
Description:
The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by thymocytes, T-helper cells, and at low levels on monocytes. CD4 acts as a receptor for HIV, and RPA-T4 blocks HIV binding and mixed lymphocyte reaction. RPA-T4 recognizes a different epitope from OKT4 and does not cross-block.
Applications Reported:
For use in flow cytometric analysis.
Applications Tested:
Pre-diluted and tested by flow cytometry on normal human peripheral blood cells. Recommended usage: 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
Fluorophore Information:
Alexa Fluor 660 emits at 690 nm, suitable for spectral cytometers and multiplexing with APC, Alexa Fluor 647, and Alexa Fluor 700.
Excitation: 662 nm / Emission: 690 nm / Laser: Red Laser
Target Information
CD4 antigen recognizes MHC class II molecules and acts as a co-receptor for HIV. Expressed mainly in T-helper cells, monocytes, macrophages, and dendritic cells. Found in thymus, lymph nodes, tonsils, spleen, and other immune-related tissues. Functions in T-cell activation and may mediate neuronal damage in immune diseases. Multiple alternatively spliced transcripts identified (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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