Thermo Fisher Scientific CD4 Monoclonal Antibody (RPA-T4), Alexa Fluor 660, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (RPA-T4), Alexa Fluor 660, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.125 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 561
• Alexa Fluor 647
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496 / 615 / 661 / 737 / 805
• Brilliant Violet 421 / 480 / 605 / 650 / 711
• eFluor 450 / 506
• FITC
• Functional Grade
• NovaFluor Blue 510 / 585 / 690
• Pacific Orange
• PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5 / PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Custom Conjugation available

Product Specific Information

Description:
The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by thymocytes, T-helper cells, and at low levels on monocytes. CD4 acts as a receptor for HIV, and RPA-T4 blocks HIV binding and mixed lymphocyte reaction. RPA-T4 recognizes a different epitope from OKT4 and does not cross-block.

Applications Reported:
For use in flow cytometric analysis.

Applications Tested:
Pre-diluted and tested by flow cytometry on normal human peripheral blood cells. Recommended usage: 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Fluorophore Information:
Alexa Fluor 660 emits at 690 nm, suitable for spectral cytometers and multiplexing with APC, Alexa Fluor 647, and Alexa Fluor 700.
Excitation: 662 nm / Emission: 690 nm / Laser: Red Laser

Target Information

CD4 antigen recognizes MHC class II molecules and acts as a co-receptor for HIV. Expressed mainly in T-helper cells, monocytes, macrophages, and dendritic cells. Found in thymus, lymph nodes, tonsils, spleen, and other immune-related tissues. Functions in T-cell activation and may mediate neuronal damage in immune diseases. Multiple alternatively spliced transcripts identified (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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