Thermo Fisher Scientific WT1-AS Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human WT1-AS(Accession Q06250), corresponding to amino acid residues V12-E62. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:WT1-AS,uniProtId:Q06250-1,ncbiNodeId:9606,antigenRange:12-62,antigenLength:92,antigenImageFileName:PA5-103316_WT1-AS_Q06250-1_Rabbit.svg,antigenImageFileNamePDP:PA5-103316_WT1-AS_Q06250-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2852661

Product Specific Information

Antibody detects endogenous levels of total WIT1.

Target Information

Wilmstumor is a neoplasm of the kidneys that usually occurs in children and is characterized by the presence of abortive tubules and glomeruli surrounded by a spindled cell stroma. The 11p13 Wilms tumor locus consists of two coordinately regulated transcripts, WT1 and WIT-1, which are mutated in Wilmstumors. Wilms tumor upstream neighbor 1 (WIT-1) is encoded by an intron-less gene upstream of the Wilms` tumor 1 (WT1) gene, which is is important for nephrogenesis and gonadal growth. The WT1 gene is bi-directionally transcribed from the same promoter region as WIT-1, which may function as an antisense regulator of WT1. WIT-1 and WT1 have the same temporal and cell-restricted expression pattern, although the expression of WIT-1 is less abundant. Methylation of the WIT-1 gene is implicated in hematologic malignancy of chemoresistant acute myeloid leukemia. Single nucleotide polymorphisms (SNPs) in the WIT-1 gene are significantly associated with focal segmental glomerulosclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.