Thermo Fisher Scientific Gliadin Monoclonal Antibody (4F3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:4,000

-

ELISA (ELISA)

1:20,000

View 1 publication 1 publication

Product Specifications

Published species

Plant

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

4F3

Immunogen

Gliadin-related peptide Lys57-Glu65- [alpha-gliadin (58-73)] (KLQPFPQPELPYPQPQ)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.98 mg/mL

Storage conditions

4° C, store in dark

Shipping conditions

Wet ice

RRID

AB_667469

Product Specific Information

HYB 314-02-02 detects the Gliadin peptide in wheat, barley, and rye samples. It does not detect the Gliadin peptide in oat samples. It detects both the deaminated and non-deaminated version of the peptide. HYB 314-02-02 has been successfully used in ELISA and Western Blotting procedures. It reacts with both the deamidated peptide and the non-deamidated peptide when coated directly in an immunoplate.

NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL.

Target Information

Celiac disease is associated with a CD4+ T-cell response to epitopes of gliadin presented by HLA-DQ2 or -DQ8 class II MHC molecules. These epitopes are present in a 33-mer peptide of wheat alpha-gliadin, residues 56-88, which is resistant to digestion and forms a substrate for tissue transglutaminase (TG2), generating the glutamic acid residues essential for binding to HLA-DQ2. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.