Thermo Fisher Scientific BAF57 Polyclonal Antibody
BAF57 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체로, WB, ICC/IF, ELISA, IP에 사용 가능. Human, Mouse, Rat 반응성. Affinity Chromatography 정제, PBS/glycerol buffer에 보관. 핵 내 단백질 검출에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 1–411 of human BAF57/SMARCE1 (NP_0030703) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.19 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2805402 |
Product Specific Information
Immunogen sequence:
MSKRPSYAPP PTPAPATQMP STPGFVGYNP YSHLAYNNYR LGGNPGTNSR VTASSGITIP KPPKPPDKPL MPYMRYSRKV WDQVKASNPD LKLWEIGKII GGMWRDLTDE EKQEYLNEYE AEKIEYNESM KAYHNSPAYL AYINAKSRAE AALEEESRQR QSRMEKGEPY MSIQPAEDPD DYDDGFSMKH TATARFQRNH RLISEILSES VVPDVRSVVT TARMQVLKRQ VQSLMVHQRK LEAELLQIEE RHQEKKRKFL ESTDSFNNEL KRLCGLKVEV DMEKIAAEIA QAEEQARKRQ EEREKEAAEQ AERSQSSIVP EEEQAANKGE EKKDDENIPM ETEETHLEET TESQQNGEEG TSTPEDKESG QEGVDSMAEE GTSDSNTGSE SNSATVEEPP TDPIPEDEKK E
Positive Samples: U-251MG, MCF-7
Cellular Location: Nucleus
Target Information
The BAF57 protein is a component of the ATP-dependent chromatin remodeling complex SWI/SNF, essential for transcriptional activation of genes repressed by chromatin. It binds to 4-way junction DNA, mimicking DNA topology at the nucleosome entry/exit. The protein contains a DNA-binding HMG domain; however, disruption of this domain does not abolish DNA-binding or nucleosome-displacement activity. Unlike most SWI/SNF complex proteins, BAF57 has no yeast counterpart.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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