Thermo Fisher Scientific P-Selectin Polyclonal Antibody
Thermo Fisher Scientific의 P-Selectin Polyclonal Antibody는 인간 CD62P 단백질을 인식하는 Rabbit IgG 기반 항체입니다. Western blot, IHC, ICC/IF에 적합하며, 고순도 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로 세포 접착 및 염증 관련 단백질 분석에 활용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Human CD62P (exact sequence proprietary) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2735612 |
Product Specific Information
- Positive Control: HUVEC
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type 1 transmembrane glycoprotein involved in cell rolling regulation.
It is stored in Weibel-Palade bodies of endothelial cells and α-granules of platelets, and rapidly translocates to the cell surface upon stimulation by thrombin, histamine, complement 5a, Ca²⁺ ionophores, or ADP.
P-selectin mediates interactions between activated endothelial cells or platelets and leukocytes, functioning as a calcium-dependent receptor binding sialylated Lewis carbohydrate antigens on neutrophils and monocytes.
It plays a key role in inflammation, atherogenesis, thrombosis, and tissue damage. Clinically, P-selectin assists in distinguishing heparin-induced thrombocytopenia with and without thrombosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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