Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (MEL-14), Alexa Fluor 700, eBioscience
마우스 CD62L(L-Selectin)을 인식하는 MEL-14 클론 단일클론항체로, Alexa Fluor 700으로 표지되어 있습니다. 유세포분석(Flow Cytometry)에 적합하며, 0.06 µg/test로 사용 가능합니다. 적혈구, 단핵구 및 림프구 하위집단 연구에 활용됩니다. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.06 µg/test | View 60 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 700, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MEL-14 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation/Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_494004 |
Available Conjugate Formats
- Alexa Fluor 561
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 615 / 737 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 786
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 510 / 555 / 585 / 610-30S / 660-40S
- NovaFluor Red 710
- NovaFluor Yellow 590 / 660
- PE
- PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436 / 600 / 702 / 780
- Request custom conjugation
Product Specific Information
Description:
The MEL-14 monoclonal antibody reacts with mouse CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, binding to glycosylated and sialylated ligands such as CD34, GlyCAM-1, and MAdCAM-1. These interactions mediate lymphocyte rolling on activated endothelium and homing to high endothelial venules (HEV) of peripheral lymphoid tissues.
Applications Reported:
This MEL-14 antibody is reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.06 µg/test. A test is defined as the amount of antibody required to stain a sample in 100 µL final volume. Optimal titration is recommended for each assay.
Fluorochrome Information:
Alexa Fluor® 700 emits at 723 nm and is excited by the red laser (633 nm). Most instruments require a 685 LP mirror and 710/20 filter.
- Excitation: 633–647 nm
- Emission: 723 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Human CD62L (L-Selectin) is a 74–95 kDa glycoprotein adhesion receptor composed of:
- C-type lectin binding domain
- EGF-like domain
- Two short consensus repeat (SCR) sequences
- Spacer, transmembrane, and cytoplasmic regions
It is constitutively expressed on most leukocytes including lymphocytes, monocytes, and polymorphonuclear cells, except for a subset of memory T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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