Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (MEL-14), Alexa Fluor 700, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Conjugate Formats

• Alexa Fluor 561
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 496 / 615 / 737 / 805
• Brilliant Violet 421 / 480 / 605 / 650 / 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510 / 555 / 585 / 610-30S / 660-40S
• NovaFluor Red 710
• NovaFluor Yellow 590 / 660
• PE
• PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 702 / 780
• Request custom conjugation

Product Specific Information

Description:
The MEL-14 monoclonal antibody reacts with mouse CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, binding to glycosylated and sialylated ligands such as CD34, GlyCAM-1, and MAdCAM-1. These interactions mediate lymphocyte rolling on activated endothelium and homing to high endothelial venules (HEV) of peripheral lymphoid tissues.

Applications Reported:
This MEL-14 antibody is reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.06 µg/test. A test is defined as the amount of antibody required to stain a sample in 100 µL final volume. Optimal titration is recommended for each assay.

Fluorochrome Information:
Alexa Fluor® 700 emits at 723 nm and is excited by the red laser (633 nm). Most instruments require a 685 LP mirror and 710/20 filter.

• Excitation: 633–647 nm
• Emission: 723 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Human CD62L (L-Selectin) is a 74–95 kDa glycoprotein adhesion receptor composed of:

• C-type lectin binding domain
• EGF-like domain
• Two short consensus repeat (SCR) sequences
• Spacer, transmembrane, and cytoplasmic regions

It is constitutively expressed on most leukocytes including lymphocytes, monocytes, and polymorphonuclear cells, except for a subset of memory T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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