Thermo Fisher Scientific APC10 Monoclonal Antibody (3E9A9)
인간 APC10 단백질을 인식하는 Mouse IgG1 단일클론 항체로, WB, IHC, ICC/IF, ELISA에 적합. 고순도 Protein G 정제, 1 mg/mL 농도, PBS buffer 및 sodium azide 함유. 세포주기 관련 단백질 연구용으로 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3E9A9 |
| Immunogen | Purified recombinant fragment of human ANAPC10 (AA: 1–185) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787246 |
Product Specific Information
MA5-31622 has been tested in indirect ELISA.
Target Information
Cell cycle regulated protein ubiquitination and degradation within subcellular domains is essential for normal mitosis progression.
APC10 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase controlling progression through mitosis and the G1 phase.
APC/C mediates degradation of anaphase inhibitors, mitotic cyclins, and spindle-associated proteins to ensure proper sequence of mitotic events.
APC10 is suggested to regulate substrate binding to the APC/C complex, similar to coactivators.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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