Thermo Fisher Scientific APC10 Monoclonal Antibody (8F1D10)
Thermo Fisher Scientific의 APC10 Monoclonal Antibody (8F1D10)는 인간 APC10 단백질을 인식하는 Mouse IgG1 항체로, WB, IHC, ICC/IF, Flow cytometry 및 ELISA에 적합합니다. Protein G로 정제된 액상 형태이며, 연구용으로 세포주기 관련 단백질 분석에 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8F1D10 |
| Immunogen | Purified recombinant fragment of human ANAPC10 (AA: 1–185) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787247 |
Product Specific Information
MA5-31623 has been tested in indirect ELISA.
Target Information
Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis.
APC10 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle.
APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins, ensuring that events of mitosis take place in proper sequence.
APC10 may regulate the binding of specific substrates to the APC/C complex, similar to that of coactivators.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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