Thermo Fisher Scientific USP13 Recombinant Rabbit Monoclonal Antibody (JU72-38)
USP13 단백질을 인식하는 Rabbit Recombinant Monoclonal Antibody로, Western blot 및 Immunoprecipitation에 최적화됨. 높은 특이성과 재현성을 제공하며, lot 간 일관성이 우수함. HEK293 세포 발현 시스템에서 생산된 동물 유래 성분 무첨가 항체.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunoprecipitation (IP) | 1:10–1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JU72-38 |
| Immunogen | Synthetic peptide within Human USP13 aa 739–788 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2810078 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone the specific antibody DNA sequences from immunoreactive rabbits, and individual clones are screened to select the best candidates for production.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
USP13 is a deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2, and USP10. It plays roles in autophagy and endoplasmic reticulum-associated degradation (ERAD).
- Regulates autophagy and p53/TP53 levels through interaction with BECN1 and USP10
- Participates in a regulatory loop involving PIK3C3/VPS34 complexes
- Recruited by nuclear UFD1 to mediate deubiquitination of SKP2
- Regulates ERAD via UBL4A deubiquitination
- Stabilizes SIAH2 by impairing its autoubiquitination
- Prefers Lys-63–linked polyubiquitin chains and shows weak deubiquitinase activity in vitro
- May cleave ISG15 in vitro (requires further validation)
Source: UniProt
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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