Thermo Fisher Scientific JMJD2B Recombinant Rabbit Monoclonal Antibody (JE36-58)
JMJD2B(KDM4B) 단백질을 인식하는 토끼 단클론 항체로, Western blot, IHC, ICC/IF에 적합. 인간 및 마우스 시료 반응성. HEK293 발현 시스템에서 생산된 재조합 항체로, Protein A 정제 및 액상 형태로 제공. 핵 내 히스톤 탈메틸화 연구에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 | — |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 | — |
| Immunocytochemistry (ICC/IF) | 1:250 | — |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JE36-58 |
| Immunogen | Synthetic peptide within Human KDM4B aa 97–146/1096 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 0.05% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3093736 |
Product Specific Information
- Positive Control: HeLa cell lysate, HCT116 cell lysate, SW480 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, human breast carcinoma tissue, HeLa, SW480
- Predicted Band Size: 122 kDa
- Subcellular Location: Nucleus
Target Information
Histone demethylase that specifically demethylates Lys-9 of histone H3, thereby playing a role in the histone code. Does not demethylate histone H3 Lys-4, H3 Lys-27, H3 Lys-36, nor H4 Lys-20. Only able to demethylate trimethylated H3 Lys-9, with a weaker activity than KDM4A, KDM4C, and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate. [UniProt]
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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