Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), Super Bright 645, eBioscience
인간 CD31(PECAM-1)을 인식하는 WM-59 단클론 항체로, Super Bright 645 형광체 결합형입니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 405 nm 레이저로 여기되어 645 nm에서 방출됩니다. 광민감성이 있으므로 암조건에서 보관해야 합니다.
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Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), Super Bright 645, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunocytochemistry (ICC/IF) | - | View 3 publications |
| Flow Cytometry (Flow) | 5 µL (0.06 µg)/test | View 17 publications |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 645 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | WM-59 (WM59) |
| Conjugate | Super Bright™ 645 |
| Excitation / Emission Max | 414 / 645 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2717093 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Request custom conjugation
Product Specific Information
The WM59 monoclonal antibody reacts with human CD31 (platelet-endothelial cell adhesion molecule-1, PECAM-1, gpIIa), a 130–140 kDa surface protein expressed by endothelial cells and at low levels on leukocytes and platelets. CD31 plays a role in adhesion, cell-cell and cell-matrix interaction, and signal transduction.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on normal human peripheral blood cells at ≤5 µL (0.06 µg)/test. A test is defined as staining a cell sample in 100 µL final volume. Cell number may range from 10⁵–10⁸ cells/test. Optimize titration for best results.
Fluorochrome Information:
Super Bright 645 is a tandem dye excited by the violet laser (405 nm) and emits at 645 nm. Recommended detection filter: 660/20 bandpass.
Usage Recommendations:
- Use Super Bright Complete Staining Buffer (Product # SB-4401) when combining multiple Super Bright dyes to reduce non-specific interactions.
- Protect vial and stained samples from light (light-sensitive dye).
- Samples can be fixed with IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C with minimal brightness loss.
Excitation: 405 nm
Emission: 645 nm
Laser: Violet Laser
Super Bright Polymer Dyes are licensed from Becton, Dickinson and Company.
Target Information
CD31 (PECAM-1) is an inhibitory coreceptor regulating T and B cell signaling via ITIM motifs. It is expressed throughout the vascular compartment, mainly at intercellular junctions. Its N-terminal Ig-like domain mediates homophilic binding, crucial for cell-cell interactions. CD31 functions in integrin-mediated adhesion, transendothelial migration, angiogenesis, apoptosis, immune regulation, macrophage phagocytosis, IgE-mediated anaphylaxis, and thrombosis.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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