Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (390), Super Bright 645, eBioscience
마우스 CD31(PECAM-1)을 인식하는 단클론 항체로, Super Bright 645 형광체가 결합된 형광유세포분석용 항체입니다. 405 nm 레이저로 여기되어 645 nm에서 방출되며, 세포 접착 및 신호전달 연구에 적합합니다. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 2 publications |
| Immunohistochemistry (IHC) | - | View 13 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 10 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 46 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 645, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 390 |
| Conjugate | Super Bright™ 645 |
| Excitation / Emission Max | 414 / 645 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2717090 |
Additional Formats Available
- Unconjugated
- APC
- Biotin
- Brilliant UV 661
- Brilliant UV 737
- Brilliant Violet 421
- Brilliant Violet 711
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 660-120S
- NovaFluor Red 685
- NovaFluor Red 710
- NovaFluor Red 725
- NovaFluor Red 755
- NovaFluor Yellow 610
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Request custom conjugation
Product Specific Information
The 390 monoclonal antibody reacts with mouse CD31 (PECAM-1, gpIIa), a 130–140 kDa surface protein expressed on endothelial cells, leukocytes, and platelets. CD31 mediates homotypic adhesion and participates in cell-cell and cell-matrix interactions and signal transduction.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse bone marrow cells at ≤0.25 µg/test (100 µL final volume). Optimal titration is recommended.
Fluorochrome Information:
Super Bright 645 is a tandem dye excited by 405 nm (violet laser) and emits at 645 nm. Recommended filter: 660/20 bandpass. Use Super Bright Complete Staining Buffer (Product # SB-4401) when combining multiple Super Bright dyes.
Handling Notes:
- Light sensitive; protect from light.
- Fixation compatible with IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333).
- Samples can be stored up to 3 days at 4°C in dark.
Excitation: 405 nm
Emission: 645 nm
Laser: Violet
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD31 (PECAM-1) is an inhibitory coreceptor regulating T and B cell signaling through ITIM motifs. It is expressed in vascular endothelial junctions and plays key roles in:
- Cell-cell adhesion and migration
- Angiogenesis and apoptosis
- Regulation of immune signaling
- Thrombosis and vascular homeostasis
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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