Thermo Fisher Scientific UBE2E3 Monoclonal Antibody (OTI4C8), TrueMAB
Thermo Fisher Scientific의 UBE2E3 Monoclonal Antibody (OTI4C8)은 인간 UBE2E3 단백질을 인식하는 Mouse IgG1 항체로, WB, ICC/IF, Flow Cytometry에 적합합니다. 무보존제, 동결건조 형태이며 재구성 후 1 mg/mL 농도로 사용 가능합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4C8 |
| Immunogen | Full length human recombinant protein of human UBE2E3 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
- For conjugation experiments, perform desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates:
- Activation: The Ub C-terminus is activated in an ATP-dependent manner by the E1 enzyme.
- Conjugation: The activated Ub is transferred to the E2 conjugating enzyme, forming a Ub-E2 intermediate.
- Ligation: The E3 ligase catalyzes the transfer of Ub from E2 to target substrates.
UBE2E3 (also known as UBCH9) catalyzes the covalent attachment of ubiquitin to proteins and regulates transepithelial sodium transport in renal cells. It may also be involved in cell growth arrest. UBE2E3 shuttles between the cytoplasm and nucleus in an IPO11-dependent manner and is ubiquitously expressed at low levels, with high expression in skeletal muscle.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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