Thermo Fisher Scientific Phospho-ROCK1 (Thr455, Ser456) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

Antibody detects endogenous levels of ROCK1 only when phosphorylated at Thr455/Ser456.

Target Information

ROCK1 is a serine/threonine kinase that regulates cell differentiation and migration. The Rho/ROCK pathway is involved in the progression of various human cancers. ROCK1 has been identified as a Caspase-3 substrate. It contributes to myosin light chain (MLC) activation through direct phosphorylation and inhibition of MLC phosphatase, resulting in increased cellular contractility necessary for apoptotic membrane blebbing.
ROCK1 consists of an amino-terminal kinase domain and a cysteine/histidine-rich inhibitory C-terminal domain within a pleckstrin-homology region, connected by a variable Rho-binding domain. During apoptosis, ROCK1 is cleaved by caspase-3 at a conserved DETD1113/G sequence, removing its inhibitory domain and leading to constitutive kinase activity. This caspase-3-mediated activation induces MLC phosphorylation and membrane blebbing, key events in apoptosis execution.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.