Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), NovaFluor Blue 510, eBioscience

Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), NovaFluor Blue 510, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496, 563, 615, 661, 737
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, FITC
• Functional Grade
• NovaFluor Blue 610-70S, Blue 660-120S
• NovaFluor Red 685, Red 700, Red 710
• NovaFluor Yellow 610, 660, 690, 700, 730
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610, PerCP-Cyanine5.5
• Super Bright 436, 600, 780
• Request custom conjugation available

Product Specific Information

The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. CD3e, together with CD3γ and CD3δ, is essential for TCR assembly, trafficking, and surface expression. CD3 is expressed by thymocytes and mature T cells. Binding of 145-2C11 to TCR induces intracellular signaling leading to activation, proliferation, or apoptosis depending on conditions.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometry of mouse splenocytes (≤1.0 µg/test, 100 µL final volume, 10⁵–10⁸ cells/test). Antibody titration recommended for optimal performance.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability analysis.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining. Add 5 µL per sample before combining with cells.

NovaFluor conjugates utilize Phiton™ technology, based on engineered nucleic acid dye structures for precise fluorescent signatures minimizing spillover and spread.

Excitation: 496 nm
Emission: 511 nm
Laser: 488 nm (Blue)

Target Information

The CD3 complex transduces antigen-recognition signals in T cells and regulates TCR surface expression. It includes CD3γ, CD3δ, CD3ε, and CD3ζ subunits, encoded on chromosome 11. CD3 is expressed on most T lymphocytes and thymocytes, but not on B or NK cells. It plays roles in TCR-induced growth arrest, survival, and proliferation. Defects in CD3 genes are linked to immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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