Thermo Fisher Scientific ACSL4 Recombinant Rabbit Monoclonal Antibody (JE56-33)
ACSL4 단백질을 인식하는 토끼 단클론 항체로, Western blot, IHC, ICC, Flow cytometry 등에 사용 가능. Human, Mouse, Rat 시료에 반응하며, HEK293 발현 시스템에서 생산된 recombinant 형식. Protein A 정제, 1 mg/mL 농도, 장기 보관 시 -20°C 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:5,000 | View 1 publication |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | – |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 | – |
| Flow Cytometry (Flow) | 1:50–1:100 | – |
Product Specifications
| Category | Specification |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JE56-33 |
| Immunogen | Recombinant protein within human FACL4 aa 561–711 (Cytoplasmic) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2911664 |
Product Specific Information
- Positive Control: NIH/3T3 cell lysate, HepG2 cell lysate, HUVEC, rat brain tissue, mouse brain tissue, HepG2
- Subcellular Location: Peroxisome membrane, Mitochondrion outer membrane, Microsome membrane, Endoplasmic reticulum membrane, Cell membrane
Target Information
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters and play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as a substrate. The absence of this enzyme may contribute to mental retardation or Alport syndrome. Alternative splicing of this gene generates two transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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