
Thermo Fisher Scientific Goat anti-Human IgG (Lambda light chain) Recombinant Secondary Antibody
인간 IgG 람다 경쇄를 특이적으로 인식하는 염소 유래 재조합 2차 항체. 높은 특이성과 로트 간 일관성 제공. WB, ICC/IF, ELISA 등 다양한 응용 가능. 동물 유래 성분 없는 포뮬레이션으로 민감도 및 재현성 향상.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:1,000 |
| ELISA | 1:1,000–1:15,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Goat / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Secondary Antibody |
| Clone | 85H45L7 |
| Immunogen | Purified Human IgG |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2930923 |
| Target | Lambda light chain |
| Antibody Form | Whole Antibody |
Product Specific Information
This Goat anti-Human IgG (Lambda Light Chain) Recombinant Secondary Antibody specifically detects human IgG lambda light chain with no cross-reactivity to kappa light chain, heavy chain, or immunoglobulins from other species.
Recombinant antibodies are produced using specific genes that encode the desired antibody. These genes are cloned into an expression vector and expressed in vitro. Advantages include:
- Enhanced specificity
- Animal origin-free formulation
- Lot-to-lot consistency
Each lot’s sensitivity and specificity are confirmed using ELISA.
It is recommended to titrate the antibody for optimal performance in the desired assay.
Target Information
Anti-Human secondary antibodies are affinity-purified reagents with well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of target molecules. Secondary antibodies offer:
- Versatility with multiple detection systems (HRP, AP, fluorescence)
- Signal amplification for improved sensitivity
Typically, secondary antibodies are generated by immunizing host animals with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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