Thermo Fisher Scientific Goat anti-Human IgG (Kappa Light Chain) Recombinant Secondary Antibody, Alexa Fluor Plus 488
Goat anti-Human IgG (Kappa Light Chain) Alexa Fluor Plus 488 형광 표지 2차 항체로, 사람 IgG Kappa 사슬에 특이적입니다. 재조합 단일클론 항체로 높은 특이성과 로트 간 일관성을 제공합니다. ICC/IF 및 Flow Cytometry에 적합하며, 4°C 암소 보관이 권장됩니다.
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Applications and Tested Dilution
| Application | Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:500 - 1:1,000 |
| Flow Cytometry (Flow) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Secondary Antibody |
| Clone | 81H59L2 |
| Immunogen | Purified Human IgG |
| Conjugate | Alexa Fluor™ Plus 488 |
| Excitation / Emission Max | 493 / 518 nm |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 6.5 |
| Contains | 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Wet ice |
| RRID | AB_2937237 |
| Target | Kappa light chain |
| Antibody Form | Whole Antibody |
Product Specific Information
This Goat anti-Human IgG (Kappa Light Chain) Recombinant Secondary Antibody specifically detects human IgG Kappa light chain with no cross-reactivity to Lambda light chain, heavy chain, or immunoglobulins from other species.
Recombinant antibodies are produced using genes encoding the desired antibody, cloned into an expression vector, and expressed in vitro. This ensures high specificity and lot-to-lot consistency.
Each lot’s sensitivity and specificity are validated by ELISA.
For optimal assay performance, titration is recommended.
Target Information
Anti-Human secondary antibodies are affinity-purified with defined specificity for human immunoglobulins. They are useful for detection, sorting, or purification of human Ig targets.
Secondary antibodies enhance versatility and sensitivity by allowing multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification through multiple binding events.
Typically, secondary antibodies are generated by immunizing host animals with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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