
Thermo Fisher Scientific SIRT5 Monoclonal Antibody (OTI1B6), TrueMAB
SIRT5 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, ICC/IF, Flow cytometry에 사용 가능. Human 시료에 반응하며, Lyophilized 형태로 제공. PBS buffer에 8% trehalose 포함, -20°C 보관 권장. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B6 |
| Immunogen | Full length human recombinant protein of human SIRT5 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
SIRT5 is a member of the sirtuin family (Sir2-like proteins), found in both prokaryotes and eukaryotes.
All sirtuins share a conserved core domain involved in NAD metabolism and possibly mono-ADP-ribosyltransferase activity.
Although the enzymatic role of sirtuins is not fully understood, studies suggest they may participate in histone deacetylation and NAD metabolism through coupled activation mechanisms involving NAD and acetylated histones.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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