Thermo Fisher Scientific LGR5 Monoclonal Antibody (OTI2A2), TrueMAB
LGR5 단백질을 인식하는 마우스 단클론 항체(OTI2A2)로, Western blot, IHC, ICC/IF, Flow cytometry, IP 등 다양한 응용에 적합합니다. 휴먼 및 마우스 반응성, carrier-free 형태로 제공되며, 고순도 친화 크로마토그래피 정제 제품입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
| Immunoprecipitation (IP) | 2 µg/500 µL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2A2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 250–550 of human LGR5 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Available Conjugated Formats
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
G protein-coupled receptor GPR49 (LGR5) is expressed in brain, skeletal muscle, placenta, and spinal cord. ESTs have been isolated from normal human brain (amygdala), embryo, placenta, germ cell, uterus, and brain libraries.
GPCRs constitute one of the largest families of signaling molecules, sharing a seven-transmembrane helix structure and existing in active and inactive forms. Ligand binding induces conformational changes that activate G-proteins and downstream effectors.
GPCR regulation and signaling are key areas of pharmacological research, with notable GPCR-targeted drugs including Zyprexa (Eli Lilly) and Claritin (Schering-Plough).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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