Thermo Fisher Scientific Vimentin Monoclonal Antibody (VIM, 3736)
Human Vimentin을 인식하는 Mouse monoclonal antibody(VIM, 3736)로, WB, IHC, Peptide Array에 사용 가능. Mouse 및 Rat에는 반응하지 않으며, PBS buffer에 보존된 액상 형태로 제공. 단백질 A/G로 정제되어 연구용으로 적합.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL | - |
| Peptide Array (Array) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | VIM, 3736 |
| Immunogen | Recombinant fragment (aa 2–466) of human Vimentin protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | –20°C or –80°C (preferred) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- This antibody does not react with Mouse or Rat.
- For Immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Vimentin is a developmentally regulated intermediate filament protein found in cells of mesenchymal origin. It is involved in the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin, along with desmin, is expressed during early cellular development and later replaced by tissue-specific intermediate filament proteins.
It plays a role in steroid synthesis by serving as a storage network for cholesterol-containing lipid droplets. Phosphorylation by protein kinase leads to filament breakdown and cytoskeletal reorganization, facilitating cholesterol transport to mitochondria and enhancing steroid synthesis.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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