Thermo Fisher Scientific CD4 Monoclonal Antibody (RPA-T4), PE-Cyanine5, eBioscience
인간 CD4 단백질을 인식하는 RPA-T4 클론 단일클론 항체로, PE-Cyanine5로 결합되어 있습니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, HIV 수용체 연구 및 T세포 아형 분석에 활용됩니다. 4°C 암소 보관, 동결 금지.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | View 55 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine5, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RPA-T4 |
| Conjugate | PE-Cyanine5 |
| Excitation/Emission Max | 568/666 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1582252 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 561
- Alexa Fluor 647
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 496
- Brilliant UV 615
- Brilliant UV 661
- Brilliant UV 737
- Brilliant UV 805
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 605
- Brilliant Violet 650
- Brilliant Violet 711
- eFluor 450
- eFluor 506
- FITC
- Functional Grade
- NovaFluor Blue 510
- NovaFluor Blue 585
- NovaFluor Blue 690
- Pacific Orange
- PE
- PE-Cyanine5.5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Request custom conjugation
Product Specific Information
Description:
The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by most thymocytes, a subpopulation of mature T-helper cells, and at low levels on monocytes. CD4 is a receptor for HIV. RPA-T4 blocks HIV binding and mixed lymphocyte reaction. The RPA-T4 antibody recognizes a different epitope than the OKT4 monoclonal antibody and does not cross-block binding.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended use: 5 µL (0.25 µg) per test (final volume 100 µL; 10⁵–10⁸ cells/test).
Light Sensitivity:
PE-Cyanine5 is sensitive to photo-induced oxidation. Protect from light.
Fixation:
Samples may be stored in IC Fixation Buffer (Product #00-8222-49) or 1-Step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark with minimal effect on brightness.
Excitation/Emission:
488–561 nm / 667 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD4 antigen is involved in MHC class II recognition and serves as a co-receptor for HIV. It is expressed mainly on T-helper lymphocytes and at lower levels on monocytes, macrophages, and dendritic cells. CD4 expression is also found in thymus, lymph nodes, tonsils, spleen, and some non-lymphoid tissues. It plays a key role in T-cell activation and may mediate neuronal damage in immune-related diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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