Thermo Fisher Scientific Phospho-CHK1 (Ser345) Polyclonal Antibody
CHK1 단백질의 Ser345 인산화 형태를 검출하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF에 적합하며 Human 및 Mouse 시료에 반응. 항원 친화 크로마토그래피로 정제된 액상 형태로, 4°C 또는 -20°C에서 보관. DNA 손상 반응 연구에 유용.
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding phospho Ser345 of CHK1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.66 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS (pH 7) with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2551977 |
Product Specific Information
- Recommended positive controls: HCT116 cells, or HCT116 treated with 30 µM cisplatin for 24 hours.
- Predicted reactivity: Mouse (100%), Rat (100%), Bovine (100%).
- IHC notes: Requires antigen retrieval using heat-mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
- Store as a concentrated solution. Centrifuge briefly before opening the vial.
Target Information
CHK1 (CHEK1) is a serine/threonine protein kinase that prevents DNA replication when genomic integrity is compromised. It plays a central role in the DNA damage-induced checkpoint network and is evolutionarily conserved.
When CHK1 function is inhibited, p53-deficient cancer cells undergo mitotic catastrophe and apoptosis, while normal cells arrest in G1 phase. Therefore, CHK1 inhibition can enhance the efficacy of DNA-damaging agents in cancer therapy, making CHK1 an attractive target for anti-cancer research.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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