Thermo Fisher Scientific Phospho-Histone H3 (Ser28) Polyclonal Antibody
Phospho-Histone H3 (Ser28) 항체로, Western blot, ICC/IF, Flow cytometry에 사용 가능. Rabbit polyclonal IgG로 다양한 종에 반응. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성 제공. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:800 |
| Flow Cytometry (Flow) | 1:400 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Fruit fly, Hamster, Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the amino terminus of histone H3 phosphorylated on Ser28 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 369 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10975400 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with other phosphorylated histones, including phospho-histone H3 (Ser10).
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome.
Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
Post-translationally, histones are modified in various ways to either directly change chromatin structure or allow binding of specific transcription factors.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modifications (e.g., methylation, acetylation, phosphorylation) that influence cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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