Thermo Fisher Scientific FGF2 Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific FGF2 Polyclonal Antibody, Biotin (PeproTech)

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human FGF-basic. Anti-Human FGF-basic-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hFGF-basic by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human FGF-basic (500-P18) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hFGF-basic.

Western Blot:
To detect Human FGF-basic by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant Human FGF-basic is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package Information:
500-P18BT-1MG will be provided as 2 × 500 µg.

Target Information

FGF2 (FGFb, fibroblast growth factor basic) belongs to the fibroblast growth factor (FGF) family and interacts with high-affinity transmembrane receptors to influence cell proliferation and tissue neovascularization.
FGF2 exists as five isoforms with distinct intracellular localizations and functions. The 18 kDa isoform is predominantly cytosolic and acts through cell surface receptors, whereas the 22, 22.5, 24, and 34 kDa isoforms are nuclear and may signal independently of transmembrane receptor pathways.
In humans, the gene is located on the q arm of chromosome 4. FGF2 is involved in limb and nervous system development, wound healing, and tumor growth.
The mRNA for FGF2 contains multiple polyadenylation sites and is alternatively translated from non-AUG and AUG initiation codons, resulting in five isoforms with distinct properties.
CUG-initiated isoforms are nuclear and responsible for intracrine effects, while the AUG-initiated form is mostly cytosolic, mediating paracrine and autocrine effects.
Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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