Thermo Fisher Scientific IL-19 Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MLRRCLISTD MHHIEESFQE IKRAIQAKDT FPNVTILSTL ETLQIIKPLD VCCVTKNLLA FYVDRVFKDH QEPNPKILRK ISSIANSFLY MQKTLRQCQE QRQCHCRQEA TNATRVIHDN YDQLEVHAAA IKSLGELDVF LAWINKNHEV MSSA

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-19. Anti-Human IL-19-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hIL-19 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-19 (500-P189) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hIL-19.

Western Blot:
To detect Human IL-19 by Western Blot, use at 0.1–0.2 µg/mL. With compatible secondary reagents, the detection limit for Recombinant Human IL-19 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Package Information:
500-P189BT-1MG will be provided as 2 × 500 µg.

Target Information

The protein encoded by this gene is a cytokine belonging to the IL10 cytokine subfamily. It is preferentially expressed in monocytes and binds the IL20 receptor complex, activating STAT3. In mice, a similar cytokine up-regulates IL6 and TNF-alpha expression and induces apoptosis, suggesting a role in inflammatory responses. Alternatively spliced transcript variants encoding distinct isoforms have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.