Thermo Fisher Scientific CDC123 Monoclonal Antibody (OTI3G8), TrueMAB
CDC123 단백질을 인식하는 Mouse monoclonal antibody (Clone OTI3G8). Western blot과 IHC(P)에서 검증됨. HEK293T 세포에서 발현된 full-length human CDC123 단백질로 면역화됨. Lyophilized 형태로 제공되며 PBS buffer에 8% trehalose 포함. 연구용 전용 시약.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3G8 |
| Immunogen | Full length human recombinant protein of human CDC123 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid dehydrogenase (BCKD) complex.
This complex includes:
- A heterotetrameric (α2β2) E1 decarboxylase
- A homo-24-meric dihydrolipoyl transacylase
- A homodimeric dihydrolipoamide dehydrogenase
E1 is a thiamine pyrophosphate (TPP)-dependent enzyme that catalyzes the irreversible first step in branched-chain amino acid oxidation.
The BCKDHB gene encodes the beta subunit of E1. The complex also includes two regulatory enzymes: a kinase and a phosphorylase.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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