Thermo Fisher Scientific CD18 (LFA-1 beta) Monoclonal Antibody (M18/2), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

-

Immunohistochemistry (IHC)

Assay-Dependent

View 4 publications 4 publications

Flow Cytometry (Flow)

0.5 µg/test

View 6 publications 6 publications

Immunoprecipitation (IP)

Assay-Dependent

-

Neutralization (Neu)

Assay-Dependent

View 2 publications 2 publications

Functional Assay (Functional)

Assay-Dependent

-

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

M18/2

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• FITC
• Functional Grade
• PE
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467138

Product Specific Information

Description: The M18/2 monoclonal antibody reacts with mouse CD18, the 90-95 kDa integrin beta 2 glycoprotein. CD18 is the common beta subunit that non-covalently associates with CD11a (alpha L), CD11b (alpha M) and CD11c (alpha X) to form LFA-1, Mac-1 and gp150/95. Heterodimers of CD18 and the a subunits are expressed broadly by all leukocytes with NK and T cells showing higher density of surface expression. Through the interactions of LFA-1, Mac-1 and gp150/95 with their counter-receptors, CD18 is involved in cellular adhesion and the immune response.

Applications Reported: The M18/2 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining. It has also been reported in blocking of CD18 in functional studies. (Please use Functional Grade purified M18/2, Product # 16-0181, in functional assays).

Applications Tested: The M18/2 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD18 integrin beta 2 subunit is a 90 kDa type I transmembrane protein expressed on all leucocytes. CD18 can combine with integrin molecules CD11a-c to form heterodimers at the cell surface, and these heterodimers are known to participate in the process of cell adhesion as well as cell-surface mediated signaling. CD18 forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits, and these integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis. Defects in the CD18 gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the CD18 protein have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.