Thermo Fisher Scientific CYP2E1 Recombinant Rabbit Monoclonal Antibody (JM10-85)
CYP2E1 단백질 검출용 재조합 토끼 단클론 항체로, 높은 특이성과 민감도를 제공하며 Western blot, IHC, ICC/IF에 적합합니다. HEK293 발현 시스템 기반, Protein A 정제, 일관된 로트 간 품질을 보장합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM10-85 |
| Immunogen | Synthetic peptide within Human CYP2E1 (aa 71–114) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809882 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
CYP2E1 is a member of the cytochrome P450 superfamily of enzymes, functioning as a monooxygenase involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids.
This enzyme localizes to the endoplasmic reticulum and is induced by ethanol, diabetic states, and starvation.
It metabolizes both endogenous substrates (ethanol, acetone, acetal) and exogenous substrates (benzene, carbon tetrachloride, ethylene glycol, nitrosamines).
Due to its diverse substrate range, CYP2E1 is implicated in processes such as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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