Thermo Fisher Scientific Nucleolin Polyclonal Antibody
Rabbit polyclonal antibody recognizing human Nucleolin (NCL). Validated for IHC (P) and ICC/IF applications. Supplied as liquid, 0.1 mg/mL, purified by antigen affinity chromatography. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human NCL. Recombinant protein control fragment (Product # RP-102502) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2790016 |
Product Specific Information
Immunogen sequence:
VLSNLSYSAT EETLQEVFEK ATFIKVPQNQ NGKSKGYAFI EFASFEDAKE ALNSCNKREI EGRAIRLELQ GPRGSPNARS QPSKTLFVKG LSEDTTEETL KESFDGSVRA RIVTDRETGS SKGFGFVDFN SEEDA
Target Information
Nucleolin (NCL) is a eukaryotic nucleolar phosphoprotein involved in the synthesis and maturation of ribosomes. It is primarily located in the dense fibrillar regions of the nucleolus. The human NCL gene consists of 14 exons and 13 introns, spanning approximately 11 kb. Intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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