Thermo Fisher Scientific Cytochrome C Monoclonal Antibody (37BA11)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.25-1 µg/mL

View 9 publications 9 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

0.5-2 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, C. elegans, Human, Mouse, Rat

Published species

Human, Mouse, Rat, Yeast

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

37BA11

Immunogen

Bovine heart Cytochrome C

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

IgG fraction

Storage buffer

HEPES buffered saline

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2533821

Product Specific Information

45-6100 was used successfully in the western blot analysis of cytochrome C using human breast cancer cell line lysates.

Positive control: Isolated mitochondria from Human, Bovine, Rat and Mouse heart; Human fibroblasts; HeLa cells.

WB: Predicted molecular weight: 14 kDa. ICC/IF: Heat induced antigen retrieval in 0.1 M Tris-HCl, 5% urea, pH 9.5 for 5 min at 95°C improves signal.

Target Information

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.