Thermo Fisher Scientific Cytochrome C Monoclonal Antibody (37BA11)
Cytochrome C 단백질 검출용 마우스 단일클론 항체. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용 가능. 미토콘드리아 및 세포자멸사 연구에 적합. Bovine heart Cytochrome C를 면역원으로 제작된 비결합형 항체.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.25–1 µg/mL | View 9 publications |
| Immunohistochemistry (IHC) | – | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | View 2 publications |
| Immunocytochemistry (ICC/IF) | 0.5–2 µg/mL | View 1 publication |
| Flow Cytometry (Flow) | 1 µg/mL | View 1 publication |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, C. elegans, Human, Mouse, Rat |
| Published Species | Human, Mouse, Rat, Yeast |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 37BA11 |
| Immunogen | Bovine heart Cytochrome C |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | IgG fraction |
| Storage Buffer | HEPES buffered saline |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2533821 |
Product Specific Information
Antibody 45-6100 was successfully used in western blot analysis of cytochrome C using human breast cancer cell line lysates.
Positive controls: Isolated mitochondria from Human, Bovine, Rat, and Mouse heart; Human fibroblasts; HeLa cells.
WB: Predicted molecular weight: 14 kDa.
ICC/IF: Heat-induced antigen retrieval in 0.1 M Tris-HCl, 5% urea, pH 9.5 for 5 min at 95°C improves signal.
Target Information
Cytochrome C is an electron-transporting protein located in the intermembrane space of mitochondria, playing a critical role in oxidative phosphorylation and ATP production.
Release of cytochrome C from mitochondria to cytosol is triggered by apoptotic stimuli. In the cytoplasm, it binds apoptotic protease activating factor, activating procaspase 9. Cytosolic cytochrome C also activates caspase 3, a key effector of apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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