Thermo Fisher Scientific V5 Tag Monoclonal Antibody, Alexa Fluor 647

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 2 publications 2 publications

Flow Cytometry (Flow)

-

View 3 publications 3 publications

ELISA (ELISA)

-

View 2 publications 2 publications

Inhibition Assays (IA)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Immunogen

sequence -Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-

Conjugate

Alexa Fluor™ 647 Alexa Fluor™ 647 Alexa Fluor™ 647

Excitation/Emission Max

650/671 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.5

Contains

2mM sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2532221

Product Specific Information

The Alexa FluorTM dyes provide for extraordinarily bright antibody conjugates. At the time of preparation, the products are certified to be free of unconjugated dyes and are tested in a cytological experiment to ensure low nonspecific staining. For Alexa FluorTM 647 the approximate fluorescence excitation (Ex) and emission (Em) maxima, in nm: Ex 650/Em 668.

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.