
Thermo Fisher Scientific EGFP/GFP/Venus Polyclonal Antibody
EGFP, GFP, Venus 단백질에 특이적인 폴리클로날 항체로, Western blot과 IHC에 사용 가능. 염색체 및 단백질 발현 연구에 적합하며, 다양한 형광 단백질 태그 검출에 활용. 동결건조 형태로 제공되며 장기 보관 시 -20°C에서 안정적.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300–1:2,000 |
| Immunohistochemistry (IHC) | 1:300–1:2,000 |
| Miscellaneous PubMed (Misc) | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Not Applicable |
| Host / Isotype | Goat / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptides from EGFP/GFP/Venus protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 100 µL of sterile water.
- Centrifuge to remove any insoluble material.
- Specificity: EGFP/GFP/Venus.
Target Information
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal.
GFP has been widely used as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms.
As a fusion tag, GFP can be used to localize proteins, study their movement, or research the dynamics of subcellular compartments.
GFP is a 27 kDa monomeric protein that forms a fluorescent pigment autocatalytically, absorbing blue light (max 395 nm) and emitting green light (peak 508 nm).
Its fluorescence is stable, species-independent, and suitable for various applications including:
- Monitoring gene expression
- Protein localization studies
- Protein-protein interaction assays (yeast two-hybrid)
- FRET experiments
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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