Thermo Fisher Scientific Phospho-eIF3b (Ser154) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody detects endogenous levels of EIF3B only when phosphorylated at Ser154.

Target Information

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:9388245, PubMed:17581632, PubMed:25849773, PubMed:27462815).
The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi, and eIF-5 to form the 43S pre-initiation complex (43S PIC).
It stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition.
The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:9388245, PubMed:17581632).
Additionally, it specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation, and apoptosis, using different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).
[UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.