Thermo Fisher Scientific RIP2 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Recommended positive control: Whole cell lysate from HeLa cells
• Store product as a concentrated solution
• Centrifuge briefly prior to opening the vial

Target Information

RIPK2 is a serine/threonine kinase involved in cell activation, proliferation, differentiation, and apoptosis. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize, and recruit RIPK2 through CARD-CARD domains. RIPK2 contributes to the tyrosine phosphorylation of ARHGEF2 via Src kinase, leading to NF-κB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes Lys-63-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2, and BIRC3. The polyubiquitinated protein mediates recruitment of MAP3K7/TAK1 to IKBKG/NEMO, inducing further Lys-63-linked polyubiquitination and activation of IKBKB/IKKB. Consequently, NF-κB is released from inhibitors and translocates into the nucleus to activate transcription of genes involved in immune response, growth control, and apoptosis protection. RIPK2 also plays a role in T-cell receptor engagement by promoting BCL10 phosphorylation and subsequent NF-κB activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.