Thermo Fisher Scientific Estrogen Receptor alpha Polyclonal Antibody
Western blot, IHC, ICC/IF 등 다양한 응용에 적합한 ERα 폴리클로날 항체. 인간, 랫트, 마우스 반응성. 합성 펩타이드 면역원 기반으로 높은 특이성과 재현성 제공. 핵 내 ERα 검출에 최적화된 연구용 항체.
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Thermo Fisher Scientific Estrogen Receptor alpha Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 8 publications |
| Immunohistochemistry (IHC) | - | View 7 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | 1–5 µg/mL | View publications |
| Immunocytochemistry (ICC/IF) | 1:1,000–1:4,000 | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues M(1) T M T L H T K A S G M A L L H Q I Q G N(21) C of human ER alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_325813 |
Product Specific Information
PA1-308 has been successfully used in Western blot, immunofluorescence, and immunohistochemistry procedures.
By Western blot, this antibody detects an ~64 kDa protein representing endogenous levels of ER alpha from MCF-7 cell extracts.
Immunohistochemical staining of ER alpha in rat prostate with PA1-308 results in primarily nuclear staining.
The PA1-308 immunogen is a synthetic peptide corresponding to residues M(1) T M T L H T K A S G M A L L H Q I Q G N(21) C of human ER alpha.
This peptide sequence is completely conserved between human, rat, and mouse and highly conserved between sheep, porcine, camel, bovine, and chicken ER alpha.
PA1-308 immunizing peptide (Cat. # PEP-036) is available for use in neutralization and control experiments.
Recombinant ER alpha expressed in baculovirus system (Cat. # RP-310) can be purchased for control experiments in Western blot and gel shift assays.
Target Information
Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors.
The estrogen receptor is a ligand-activated transcription factor that, when bound to estrogen, undergoes a conformational change allowing dimerization and binding to estrogen response elements (ERE) in DNA.
ER can regulate gene transcription positively or negatively through coactivator or corepressor recruitment.
There are two forms of the estrogen receptor, alpha and beta, encoded by ESR1 and ESR2 genes.
At least four estrogen receptor-alpha isoforms are known (Isoform 1: 66 kDa, Isoform 2: 53 kDa, Isoform 3: 47 kDa, Isoform 4: 35 kDa).
Estrogen receptors are widely expressed and essential for sexual development, reproductive function, and bone health.
They are implicated in diseases such as breast cancer, endometrial cancer, and osteoporosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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