Thermo Fisher Scientific Estrogen Receptor alpha Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 8 publications 8 publications

Immunohistochemistry (IHC)

-

View 7 publications 7 publications

Immunohistochemistry (Frozen) (IHC (F))

1-5 µg/mL

-

Immunocytochemistry (ICC/IF)

1:1,000-1:4,000

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues M(1) T M T L H T K A S G M A L L H Q I Q G N(21) C of human ER alpha. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Estrogen Receptor alpha,uniProtId:P03372-1,ncbiNodeId:9606,antigenRange:1-21,antigenLength:595,antigenImageFileName:PA1-308_Estrogen_Receptor_alpha_P03372-1_Rabbit.svg,antigenImageFileNamePDP:PA1-308_Estrogen_Receptor_alpha_P03372-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325813

Product Specific Information

PA1-308 has been successfully used in Western blot, immunofluorescence and immunohistochemistry procedures. By Western blot, this antibody detects an ~64 kDa protein representing endogenous levels of ER alpha from MCF-7 cell extracts. Immunohistochemical staining of ER alpha in rat prostate with PA1-308 results in primarily nuclear staining.

The PA1-308 immunogen is a synthetic peptide corresponding to residues M(1) T M T L H T K A S G M A L L H Q I Q G N(21) C of human ER alpha. This peptide sequence is completely conserved between human, rat and mouse and is highly conserved between sheep, porcine, camel, bovine and chicken ER alpha. PA1-308 immunizing peptide (Cat. # PEP-036) is available for use in neutralization and control experiments.

Recombinant ER alpha expressed in baculovirus system (Cat. # RP-310) can be purchased for control experiments in Western blot and gel shift assays.

Target Information

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.