Thermo Fisher Scientific ATF6 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Positive Samples: MCF7, Mouse brain, Mouse kidney, HeLa, Rat liver

Immunogen Sequence:
PKTQTNSSVP AKTIIIQTVP TLMPLAKQQP IISLQPAPTK GQTVLLSQPT VVQLQAPGVL PSAQPVLAVA GGVTQLPNHV VNVVPAPSAN SPVNGKLSVT KPVLQSTMRN V

Target Information

ATF6 is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. It binds DNA on the 5'-CCAC[GA]-3' half of the ER stress response element (ERSE) and ERSE II. Binding to ERSE requires NF-Y. ATF6 can form homodimers or heterodimers with ATF6 beta and interacts with NF-YC, GTF2I, YY1, and SRF. Under ER stress, the cleaved N-terminal cytoplasmic domain translocates into the nucleus, where it functions as a transcription factor. The basic domain acts as a nuclear localization signal, and the basic leucine-zipper domain mediates binding to ERSE. The 50 kDa fragment is released by sequential cleavage via site-1 and site-2 proteases. ATF6 is N-glycosylated, phosphorylated by MAPK14/P38MAPK, and belongs to the bZIP family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.