Thermo Fisher Scientific ATF6 Polyclonal Antibody
ATF6 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF, ELISA에 사용 가능. 인간, 마우스, 랫트 반응성. 친화 크로마토그래피로 정제된 액상 항체이며, -20°C에서 보관. 연구용으로 ER 스트레스 관련 단백질 분석에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 190–300 of human ATF6 (NP_0313742) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901125 |
Product Specific Information
Positive Samples: MCF7, Mouse brain, Mouse kidney, HeLa, Rat liver
Immunogen Sequence:
PKTQTNSSVP AKTIIIQTVP TLMPLAKQQP IISLQPAPTK GQTVLLSQPT VVQLQAPGVL PSAQPVLAVA GGVTQLPNHV VNVVPAPSAN SPVNGKLSVT KPVLQSTMRN V
Target Information
ATF6 is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. It binds DNA on the 5'-CCAC[GA]-3' half of the ER stress response element (ERSE) and ERSE II. Binding to ERSE requires NF-Y. ATF6 can form homodimers or heterodimers with ATF6 beta and interacts with NF-YC, GTF2I, YY1, and SRF. Under ER stress, the cleaved N-terminal cytoplasmic domain translocates into the nucleus, where it functions as a transcription factor. The basic domain acts as a nuclear localization signal, and the basic leucine-zipper domain mediates binding to ERSE. The 50 kDa fragment is released by sequential cleavage via site-1 and site-2 proteases. ATF6 is N-glycosylated, phosphorylated by MAPK14/P38MAPK, and belongs to the bZIP family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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